Detection Modes |
Fluorescence Intensity FRET Fluorescence Polarization AlphaScreen®/AlphaLISA®/AlphaPlexTM Luminescence(flash and glow) BRET Time-Resolved Fluorescence - including TR-FRET UV/Vis absorbance spectra |
Measurement Modes |
Top and bottom reading Endpoint and kinetic measurements Sequential multi-excitation measurements Sequential multi-emission measurements Spectral scanning (fluorescence, luminescence, absorbance) Ratiometric measurements Well scanning |
Microplate Formats |
6- to 1536-well plates, user-definable LVis Plate with 16 low volume microspots (2 μL) |
Microplate Carrier |
Robot compatible |
Light Sources |
High energy xenon flash lamp Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM |
Detectors |
Low noise photomultiplier tube CCD spectrometer |
Wavelength Selectiondual |
Dual Linear Variable Filter (LVF) Monochromators™ Linear Variable Dichroic Mirror Separates Ex & Em LVF Monochromators Optical filters: Ex and Em slides hold 4 filters each LVF Monochromators + optical filters Use one for Ex and the other for Em UV/Vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well |
Optical Filters |
Excitation and emission slides for 4 filters each |
Optical Path Guides |
Top and bottom: Enclosed, free-air optical light path guided by motor-driven mirrors and dichroics |
Z-Adjustment |
Automatic focal height adjustment (0.1 mm resolution) |
Spectral Range |
Filters |
240 - 750 nm or 240 - 900 nm for FI, FP, TRF 240 - 750 nm for LUM |
LVF Monochromators™ |
320 - 850 nm for FI 320 - 750 nm for LUM |
Linear Variable Dichroic |
340 - 740 nm for FI, LUM |
Spectrometer |
220 - 1000 nm for ABS |
Sensitivity* |
FI Filters (top) |
< 0.15 pM (< 3 amol/well Fluorescein, 384sv, 20 μL) |
FI Filters (bottom) |
< 1.0 pM (< 50 amol/well Fluorescein, 384g, 50 μL) |
FI Monochromator (top) |
< 0.35 pM (< 7 amol/well Fluorescein, 384sv, 20 μL) |
FI Monochromator (bottom) |
< 3.0 pM (< 150 amol/well Fluorescein, 384g, 50 μL) |
FP |
< 0.5 mP SD at 1 nM Fluorescein (384sv, 20 μL) |
HTRF® (black and white microplates) |
Reader Control Kit (Eu) after 18h (384sv, 20 μL) Delta F > 880 % (High Calibrator) Delta F > 30 % (Low Calibrator) |
TRF |
< 20 fM Europium, 384, 80 μL |
LUM |
< 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL) Dynamic Range: 9 decades |
AlphaScreen® with Laser |
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL) |
ABS with Spectrometer |
Full spectrum captured in < 1 s / well Selectable spectral resolution: 1, 2, 5, and 10 nm OD range: 0 - 4 OD Accuracy: < 1% at 2 OD Precision: < 0.5% at 1 OD and < 0.8% at 2 OD |
Read Times |
Flying mode (1 flash) |
8 s (96), 15 s (384), 28 s (1536) |
10 flashes |
19 s (96), 57 s (384), 3 min 4 s (1536) |
Reagent Injection |
Up to 2 built-in reagent injectors Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL) Variable injection speed up to 420 µL / s Reagent back flushing |
Shaking |
Linear, orbital, and double-orbital with user-definable time and speed |
Incubation |
+3 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer. |
Software |
Integrated fluorophore library Multi-user Reader Control and MARS Data Analysis Software included FDA 21 CFR Part 11 compliant |
Dimensions |
Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg |
Optional Accessories
|
LVis Plate |
Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples.
Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy
Dimensions: Conforms to SBS standards for microplates. |
Atmospheric Control Unit (ACU) |
Actively regulates O2 and CO2 - 0.1-20% |
Stacker |
Magazines for up to 50 plates - continuous loading feature |
THERMOstar |
Microplate Incubator and Shaker |
Optical Filters |
Excitation and emission slides for 4 filters each |
Upgrades |
Please contact your local DSS representative for upgrades including options such as detection modes, reagent injectors, etc. |
* |
LOD = 3 x SD (20 blanks) / slope (6 pt std curve) AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C Microplates:
- White for LUM, AlphaScreen®, TRF
- Black for FI, FP
- Clear for ABS
96 = 96-well microplates 384sv = 384-well small volume microplates 384g = 384-well glass bottom microplates 1536 = 1536-well microplates |