BMG Labtech CLARIOstar® Microplate Reader

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The CLARIOstar® is BMG LABTECH’s most flexible microplate reader, equipped with revolutionary LVF monochromator technology.Versatile, high-performance microplate reader. With its triple technology - monochromators, spectrometer, and filters - it does not compromise on sensitivity or flexibility.
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BMG Labtech CLARIOstar® Microplate Reader

Description

The CLARIOstar® is BMG LABTECH's newest microplate reader. It's a versatile, high-performance microplate reader equipped with BMG LABTECH's revolutionary LVF monochromator technology. Thanks to the triple technologies available - advanced LVF Monochromators, highly sensitive filters, and an ultra-fast UV/Vis spectrometer - it does not compromise on sensitivity or flexibility. It is a modular microplate reader with up to eight different detection modes: fluorescence intensity (including FRET), fluorescence polarization, luminescence (including BRET), UV/Vis absorbance, time-resolved fluorescence (including TR-FRET), and AlphaScreen®/AlphaLISA®. The CLARIOstar® Microplate Reader delivers high performance in all detection modes but its versatility does not end there. The CLARIOstar® is also ideal for assay development.

Anything is possible. Any wavelength. Any bandwidth. Any assay.

NEW: The CLARIOstar® is now available with an Atmospheric Control Unit for all your live cell-based assays!

What makes this instrument so special is that it is the only plate reader capable of taking readings immediately after trigger injections. It accomplishes this feat using the SPARCL Assay Protocol. SPARCL assays have the ability to start reading reactions directly after the injection step so they can record the spike that happens immediately after sample is injected.

SPARCL refers to an immunoassay that does not require washing steps. An analyte in a solution is detected by binding two antibodies: one is coupled to horse radish peroxidase (HRP), the other is coupled to the HRP substrate Acridan. In the presence of the analyte, binding of both antibodies brings the enzyme and substrate into proximity. The enzymatic reaction is started by the addition of H2O2, which immediately produces a light proportional to the amount of analyte. Thus, SPARCL assays begin reading when the trigger solution is injected.

Features:

  • Increased sensitivity over conventional monochromators
  • Continuously adjustable bandwidths (8 to 100 nm) and wavelengths (320 to 850 nm)
  • Combined use of LVF monochromators and filters in the same measurement
  • Full-spectrum UV/Vis absorbance measurements in less than 1 second
  • Dedicated, high-energy laser for AlphaScreen® and AlphaLISA®
  • Automated focal Z-height adjustment for top and bottom readings
  • Includes multi-user control and MARS data analysis software with integrated fluorophore library
  • Compatible with LVis Plate and stacker

Specifications

CLARIOstar® Microplate Reader Specifications

Detection Modes Fluorescence Intensity
FRET
Fluorescence Polarization
AlphaScreen®/AlphaLISA®/AlphaPlexTM
Luminescence(flash and glow)
BRET
Time-Resolved Fluorescence - including TR-FRET
UV/Vis absorbance spectra
Measurement Modes Top and bottom reading
Endpoint and kinetic measurements
Sequential multi-excitation measurements
Sequential multi-emission measurements
Spectral scanning (fluorescence, luminescence, absorbance)
Ratiometric measurements
Well scanning
Microplate Formats 6- to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier Robot compatible
Light Sources High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM
Detectors Low noise photomultiplier tube
CCD spectrometer
Wavelength Selectiondual Dual Linear Variable Filter (LVF) Monochromators™

Linear Variable Dichroic Mirror
Separates Ex & Em LVF Monochromators
Optical filters:
Ex and Em slides hold 4 filters each
LVF Monochromators + optical filters
Use one for Ex and the other for Em
UV/Vis absorbance spectrometer:
Full spectra or 8 distinct wavelengths in < 1 sec/well
Optical Filters Excitation and emission slides for 4 filters each
Optical Path Guides Top and bottom: Enclosed, free-air optical light path guided by motor-driven mirrors and dichroics
Z-Adjustment Automatic focal height adjustment (0.1 mm resolution)
Spectral Range Filters 240 - 750 nm or 240 - 900 nm for FI, FP, TRF
240 - 750 nm for LUM
LVF Monochromators™ 320 - 850 nm for FI
320 - 750 nm for LUM
Linear Variable Dichroic 340 - 740 nm for FI, LUM
Spectrometer 220 - 1000 nm for ABS
Sensitivity* FI Filters
(top)
< 0.15 pM (< 3 amol/well Fluorescein, 384sv, 20 μL)
FI Filters
(bottom)
< 1.0 pM (< 50 amol/well Fluorescein, 384g, 50 μL)
FI Monochromator
(top)
< 0.35 pM (< 7 amol/well Fluorescein, 384sv, 20 μL)
FI Monochromator
(bottom)
< 3.0 pM (< 150 amol/well Fluorescein, 384g, 50 μL)
FP < 0.5 mP SD at 1 nM Fluorescein (384sv, 20 μL)
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 880 % (High Calibrator)
Delta F > 30 % (Low Calibrator)
TRF < 20 fM Europium, 384, 80 μL
LUM < 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL)
Dynamic Range: 9 decades
AlphaScreen®
with Laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
ABS
with Spectrometer
Full spectrum captured in < 1 s / well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 - 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Read Times Flying mode (1 flash) 8 s (96), 15 s (384), 28 s (1536)
10 flashes 19 s (96), 57 s (384), 3 min 4 s (1536)
Reagent Injection Up to 2 built-in reagent injectors
Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)
Variable injection speed up to 420 µL / s
Reagent back flushing
Shaking Linear, orbital, and double-orbital with user-definable time and speed
Incubation +3 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
Software Integrated fluorophore library
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Dimensions Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg

Optional Accessories

LVis Plate Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples.

Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy

Dimensions: Conforms to SBS standards for microplates.
Atmospheric Control Unit (ACU) Actively regulates O2 and CO2 - 0.1-20%
Stacker Magazines for up to 50 plates - continuous loading feature
THERMOstar Microplate Incubator and Shaker
Optical Filters Excitation and emission slides for 4 filters each
Upgrades Please contact your local DSS representative for upgrades including options such as detection modes, reagent injectors, etc.
* LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates:
  • White for LUM, AlphaScreen®, TRF
  • Black for FI, FP
  • Clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates

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