BMG Labtech CLARIOstar® Plus Microplate Reader
Description
The CLARIOstar® is BMG LABTECH's newest microplate reader. It's a versatile, high-performance microplate reader equipped with BMG LABTECH's revolutionary LVF monochromator technology. Thanks to the triple technologies available - advanced LVF Monochromators, highly sensitive filters, and an ultra-fast UV/Vis spectrometer - it does not compromise on sensitivity or flexibility. It is a modular microplate reader with up to eight different detection modes: fluorescence intensity (including FRET), fluorescence polarization, luminescence (including BRET), UV/Vis absorbance, time-resolved fluorescence (including TR-FRET), and AlphaScreen®/AlphaLISA®. The CLARIOstar® Microplate Reader delivers high performance in all detection modes but its versatility does not end there. The CLARIOstar® is also ideal for assay development.
Anything is possible. Any wavelength. Any bandwidth. Any assay.
NEW: The CLARIOstar® is now available with an Atmospheric Control Unit for all your live cell-based assays!
What makes this instrument so special is that it is the only plate reader capable of taking readings immediately after trigger injections. It accomplishes this feat using the SPARCL Assay Protocol. SPARCL assays have the ability to start reading reactions directly after the injection step so they can record the spike that happens immediately after sample is injected.SPARCL refers to an immunoassay that does not require washing steps. An analyte in a solution is detected by binding two antibodies: one is coupled to horse radish peroxidase (HRP), the other is coupled to the HRP substrate Acridan. In the presence of the analyte, binding of both antibodies brings the enzyme and substrate into proximity. The enzymatic reaction is started by the addition of H2O2, which immediately produces a light proportional to the amount of analyte. Thus, SPARCL assays begin reading when the trigger solution is injected.
Features:
- Increased sensitivity over conventional monochromators
- Continuously adjustable bandwidths (8 to 100 nm) and wavelengths (320 to 850 nm)
- Combined use of LVF monochromators and filters in the same measurement
- Full-spectrum UV/Vis absorbance measurements in less than 1 second
- Dedicated, high-energy laser for AlphaScreen® and AlphaLISA®
- Automated focal Z-height adjustment for top and bottom readings
- Includes multi-user control and MARS data analysis software with integrated fluorophore library
- Compatible with LVis Plate and stacker
Specifications
CLARIOstar® Microplate Reader Specifications
Detection Modes | Fluorescence Intensity FRET Fluorescence Polarization AlphaScreen®/AlphaLISA®/AlphaPlexTM Luminescence(flash and glow) BRET Time-Resolved Fluorescence - including TR-FRET UV/Vis absorbance spectra |
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Measurement Modes | Top and bottom reading Endpoint and kinetic measurements Sequential multi-excitation measurements Sequential multi-emission measurements Spectral scanning (fluorescence, luminescence, absorbance) Ratiometric measurements Well scanning |
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Microplate Formats | 6- to 1536-well plates, user-definable LVis Plate with 16 low volume microspots (2 μL) |
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Microplate Carrier | Robot compatible | |
Light Sources | High energy xenon flash lamp Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM |
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Detectors | Low noise photomultiplier tube CCD spectrometer |
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Wavelength Selectiondual | Dual Linear Variable Filter (LVF) Monochromators™ Linear Variable Dichroic Mirror Separates Ex & Em LVF Monochromators Optical filters: Ex and Em slides hold 4 filters each LVF Monochromators + optical filters Use one for Ex and the other for Em UV/Vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well |
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Optical Filters | Excitation and emission slides for 4 filters each | |
Optical Path Guides | Top and bottom: Enclosed, free-air optical light path guided by motor-driven mirrors and dichroics | |
Z-Adjustment | Automatic focal height adjustment (0.1 mm resolution) | |
Spectral Range | Filters | 240 - 750 nm or 240 - 900 nm for FI, FP, TRF 240 - 750 nm for LUM |
LVF Monochromators™ | 320 - 850 nm for FI 320 - 750 nm for LUM |
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Linear Variable Dichroic | 340 - 740 nm for FI, LUM | |
Spectrometer | 220 - 1000 nm for ABS | |
Sensitivity* | FI Filters (top) |
< 0.15 pM (< 3 amol/well Fluorescein, 384sv, 20 μL) |
FI Filters (bottom) |
< 1.0 pM (< 50 amol/well Fluorescein, 384g, 50 μL) | |
FI Monochromator (top) |
< 0.35 pM (< 7 amol/well Fluorescein, 384sv, 20 μL) | |
FI Monochromator (bottom) |
< 3.0 pM (< 150 amol/well Fluorescein, 384g, 50 μL) | |
FP | < 0.5 mP SD at 1 nM Fluorescein (384sv, 20 μL) | |
HTRF® (black and white microplates) |
Reader Control Kit (Eu) after 18h (384sv, 20 μL) Delta F > 880 % (High Calibrator) Delta F > 30 % (Low Calibrator) |
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TRF | < 20 fM Europium, 384, 80 μL | |
LUM | < 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL) Dynamic Range: 9 decades |
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AlphaScreen® with Laser |
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL) | |
ABS with Spectrometer |
Full spectrum captured in < 1 s / well Selectable spectral resolution: 1, 2, 5, and 10 nm OD range: 0 - 4 OD Accuracy: < 1% at 2 OD Precision: < 0.5% at 1 OD and < 0.8% at 2 OD |
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Read Times | Flying mode (1 flash) | 8 s (96), 15 s (384), 28 s (1536) |
10 flashes | 19 s (96), 57 s (384), 3 min 4 s (1536) | |
Reagent Injection | Up to 2 built-in reagent injectors Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL) Variable injection speed up to 420 µL / s Reagent back flushing |
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Shaking | Linear, orbital, and double-orbital with user-definable time and speed | |
Incubation | +3 °C above ambient up to 45 °C or 65 °C The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer. |
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Software | Integrated fluorophore library Multi-user Reader Control and MARS Data Analysis Software included FDA 21 CFR Part 11 compliant |
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Dimensions | Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg | |
Optional Accessories | ||
LVis Plate | Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples. Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy Dimensions: Conforms to SBS standards for microplates. |
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Atmospheric Control Unit (ACU) | Actively regulates O2 and CO2 - 0.1-20% | |
Stacker | Magazines for up to 50 plates - continuous loading feature | |
THERMOstar | Microplate Incubator and Shaker | |
Optical Filters | Excitation and emission slides for 4 filters each | |
Upgrades | Please contact your local DSS representative for upgrades including options such as detection modes, reagent injectors, etc. | |
* | LOD = 3 x SD (20 blanks) / slope (6 pt std curve) AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C Microplates:
384sv = 384-well small volume microplates 384g = 384-well glass bottom microplates 1536 = 1536-well microplates |