Automated Fluorescent Cell Counting in Cell Counting Made Simple
The Cellometer Auto X4 utilizes bright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.
5x Faster than Manual Counting: Load sample, view image, count cells, and obtain results in < 60 seconds
The Cellometer Auto X4 Allows Hemacytometer Users to:
Fluorescent dyes that stain DNA can be used to identify nucleated cells in a mixed cell population. Acridine orange (AO) is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence when imaged with the Cellometer Auto X4.
Because mature mammalian red blood cells don't contain nuclei, only mononuclear cells produce a fluorescent signal. Fluorescent-positive nucleated cells are easily counted. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step.
Trypan Blue Viability
The Cellometer Auto X4 simultaneously calculates cell concentration and % viability for cultured cells stained with trypan blue.
Trypan blue viability is a dye exclusion method that utilizes membrane integrity to identify dead cells. The dye is unable to penetrate healthy cells, so they remain unstained. Dead cells have a compromised cell membrane that is permeable to the trypan blue dye. Dead cells are stained blue and display as dark cells in the Cellometer software with bright field imaging.
Within 30 seconds, the Cellometer Auto X4 instrument reports:
Counting Chambers: No Washing or Contamination
Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette.
The chamber is inserted into the Cellometer cell counter and the cells are imaged. This simple sample loading and analysis method is ideal for fragile cells.
The disposable Cellometer Cell Counting Chambers offer several key advantages: