Nexcelom Cellometer Auto X4 Cell Counter
Description
Automated Fluorescent Cell Counting in Cell Counting Made Simple
The Cellometer Auto X4 utilizes bright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.
5x Faster than Manual Counting: Load sample, view image, count cells, and obtain results in < 60 seconds
The Cellometer Auto X4 Allows Hemacytometer Users to:
- Increase throughput
- Improve consistency
- Ensure all data is correctly captured
- Count difficult cells (clumpy, irregular-shaped)
- Eliminate judgment errors, miscounts, and user-to-user variability
Fluorescent Analysis
Fluorescent dyes that stain DNA can be used to identify nucleated cells in a mixed cell population. Acridine orange (AO) is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence when imaged with the Cellometer Auto X4.
Because mature mammalian red blood cells don't contain nuclei, only mononuclear cells produce a fluorescent signal. Fluorescent-positive nucleated cells are easily counted. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step.
Trypan Blue Viability
The Cellometer Auto X4 simultaneously calculates cell concentration and % viability for cultured cells stained with trypan blue.
Trypan blue viability is a dye exclusion method that utilizes membrane integrity to identify dead cells. The dye is unable to penetrate healthy cells, so they remain unstained. Dead cells have a compromised cell membrane that is permeable to the trypan blue dye. Dead cells are stained blue and display as dark cells in the Cellometer software with bright field imaging.
Within 30 seconds, the Cellometer Auto X4 instrument reports:
- Live cell concentration
- Percent viability
- Mean live cell diameter
- Live and dead cell number
Counting Chambers: No Washing or Contamination
Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette.
The chamber is inserted into the Cellometer cell counter and the cells are imaged. This simple sample loading and analysis method is ideal for fragile cells.
The disposable Cellometer Cell Counting Chambers offer several key advantages:
- Time savings no washing
- No risk of cross-contamination
- Reduced biohazard risk to users
- Controlled sample volume
- Most affordable automated counting consumables
Features
Features & Benefits of the Cellometer Auto X4
- Nucleated Cell Counting in
- Trypan Blue Viability
- Fluorescent Viability Determination
- 1-Step Yeast Concentration & Viability
- Analysis of Clumpy and Irregular-Shaped Cells
- Algae Concentration using Auto X4 (10x)
- Exclusion of Debris & Size-Based Counting
- View, Print and Save Cell Images and Data
- User-Changeable Fluorescence Optics Modules
- Counting Chambers - No Washing or Contamination
- Dedicated On-line and On-site Support
- Auto X4 Accessories
Specifications
Easily Export to Excel for additional data formatting or sharing. Images and data tables can be pasted into PowerPoint presentations or submitted for publication.
Easily Save data and images to a network or local computer.
Print directly from the software screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images.
User-Changeable Fluorescence Optics Modules
The Cellometer Auto X4 Cell Counter features a user-changeable fluorescence optics module enabling detection from UV to far-red. This enables the Cellometer Auto X4 to be shared by users performing a variety of assays for nucleated cell count, metabolic activity, and fluorescent viability.
Easily switch optics modules for detection of a wide range of dyes and fluorophores:
375/450nm: DAPI, Hoechst
470/535nm: Acridine Orange (AO), Calcein, FITC
527/595nm: Ethidium Bromide (EB), Propidium Iodide (PI)
470/610nm: Chlorophyll
630/695nm: Allophycocyanin (APC)
Dedicated On-line and On-site Applications Support
Experienced Nexcelom Technical Support Specialists are available from 9am to 5pm EST for phone and on-line support and can assist with:
- Creation of new cell types
- Optimization of counting parameters
- Troubleshooting
- Training of new users
- Installation of a new Cellometer Auto X4 Cell Counter
The help button at the bottom right of the Cellometer Auto T4 software screen gives users instant access to:
- Software features and instructions
- On-line tutorials and training videos
- Submission of a Support Ticket
Clicking "Submit a Support Ticket" launches a convenient on-line form that is submitted directly to Nexcelom Technical Support
- T4 instrument information fields are automatically populated
- Images are easily attached for troubleshooting and applications support
- A Nexcelom Specialist can assume remote control of the Cellometer Auto X4 Cell Counter for troubleshooting and training